Application

Anti-Astrin, clone C-1, Cat. No. MABN2487, is a highly specific mouse monoclonal antibody that targets Sperm-associated antigen 5 and has been tested for use in Immunocytochemistry and Western Blotting.

Research CategoryNeuroscience

Western Blotting Analysis: A representative lot detected Astrin in Western Blotting applications (Yang, Y.C., et. al. (2006). Biochem Biophys Res Commun. 343(2):428-34).

Immunocytochemistry Analysis: A representative lot detected Astrin in Immunocytochemistry applications (Schmidt, J.C., et. al. (2010). J Cell Biol. 191(2):269-80; Yang, Y.C., et. al. (2006). Biochem Biophys Res Commun. 343(2):428-34; Kern, D.M., et. al. (2016). J Cell Biol. 213(3):315-28).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sperm-associated antigen 5 (UniProt: Q96R06; also known as Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126) is encoded by the SPAG5 gene (Gene ID: 10615) in human. Astrin is a homodimeric protein with a globular head domain and a long stalk. It is an essential component of the mitotic spindle. Astrin is highly expressed in testis and is detected at low levels in placenta, liver, pancreas, thymus and colon. Its expression is cell cycle-regulated, with an increase from prophase to cytokinesis and return to basal levels at the next G1 phase. In non-dividing cells Astrin is associated with microtubules unless under cellular stress, then it is localized in stress granules. In non-mitotic cells, upon stress induction, it inhibits mTORC1 association and recruits the mTORC1 component RPTOR to stress granules, thereby preventing mTORC1 hyperactivation-induced apoptosis. Astrin is required for normal chromosome segregation and progression into anaphase. It is also required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, it promotes stable microtubule-kinetochore attachments. Depletion of astrin in cells is shown to result in the activation of p53-dependent apoptosis that elevates Bax expression and increases Caspase-3 activity. Astrin can be phosphorylated at multiple sites by Aurora kinase A. (Ref.: Yang, YC., et al. (2006). Biochem. Biophys. Res. Commun. 343(2); 428-434).

Immunogen

His-tagged recombinant fragment corresponding to the first 952 amino acids from human Sperm-associated antigen 5 (Astrin).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Astrin in HeLa cell lysate.

Specificity

Clone C-1 specifically detects human Sperm-associated antigen 5 (Astrin). It detects both splice variants (130 and 140 kDa).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 and 140 kDa doublet; 134.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).